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The analyte is divided by a natural solvent gradient and quantified by an Agilent Ultivo triple quadrupole mass spectrometer working in several effect monitoring (MRM) mode. The quantitative MRM transitions had been m/z 762→234 and m/z 765→234 for trabectedin and its particular d3-labeled derivative, respectively. The reduced limit of quantification (LLOQ) was 0.01 ng/mL additionally the assay was linear up to 2.5 ng/mL. The intra- and inter-day relative error ranged from 1.19 % to 8.52 per cent, as the gener
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