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Melanin localization can be assessed ex vivo and in vivo having its distinctive optical properties. Melanin displays a characteristic Raman spectrum band shape and discernible near-infrared excited (NIR) fluorescence. Nonetheless, a detailed evaluation of this abilities of depth-resolved confocal Raman and fluorescence microspectroscopy into the analysis of melanin distribution in the man skin is lacking. Here we demonstrate the way the fraction of melanin at various depths into the man epidermis in vivo coul

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