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A novel method of the accelerated equilibration of 18O between CO2 and H2O for the measurement of the 18O/16O isotope ratios in aqueous samples with natural isotope abundances is presented. This rapid equilibrium method is based on the in vitro application of the enzyme carbonic anhydrase (CA). The CA from bovine erythrocytes was adsorptively fixed to 3-mm glass beads with an etched surface. After the addition of this carrier-fixed CA catalyst to the water sample, the isotope equilibrium was already reached after 1 h. The previously used no
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