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In addition, the triple mutant displayed aberrant localization of the DAG sensor after 5 h of growth resumption from stationary phase. Manipulation of DAG amounts by overexpression of the DAG kinase Dgk1, affected localization of the DAG probe and impacted fitness regarding the triple mutant. Altogether these results link LD usage to tubular ER expansion as a gateway of lipid precursors that usually gather in vacuolar linked membranes or other inner compartments. Moreover, conversion of DAG to p
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