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In this work, we explored the normal variation in threshold to Al3+ between high and reduced tolerant Brachiaria species and characterized their transcriptional distinctions during stress. We identified three QTLs (quantitative trait loci) connected with root vigour during Al3+ tension in their crossbreed progeny. By integrating these outcomes with a new Brachiaria reference genome, we identified 30 genetics putatively responsible for Al3+ tolerance in Brachiaria. We noticed differ
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