https://www.selleckchem.com/pr....oducts/LY2228820.htm
To observe alveolar macrophages (AMs) in the microenvironment of patients with non-small cell lung cancer (NSCLC). 20 NSCLC patients received bronchoalveolar lavage, and the bronchial alveolar lavage fluid (BALF) was collected. The phenotypes of AMs were detected by the opal multiplex immunofluorescence assay (mIF), flow cytometry, and western blot. AMs could easily be made into paraffin sections after agar pre-embedding. The mIF results showed that AMs highly expressed M1-type marker CD86, and M2-type marker CD163 under PerkinElmer V
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