Protein detection by western blot
The molecules that have been separated are moved or "blotted" onto a second matrix, which is usually made of nitrocellulose or polyvinylidene difluoride. The membrane is blocked so that antibodies can't bind to its surface in a way that isn't what they are supposed to do. Often, the secondary antibody is bound to an enzyme, which, when mixed with the right substrate, makes a signal that can be picked up.
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