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Consequently, this study had been carried out to determine the prevalence of HEV in environmental seas in urban and residential district regions in Germany. HEV was monitored in liquid examples making use of quantitative RT-PCR (RT-qPCR) and nested RT-PCR without or with virus concentration via polyethylene glycol precipitation or ultracentrifugation. By RT-qPCR, 84-100% of influent types of wastewater therapy plants had been positive for HEV RNA. Genotypes HEV-3c and 3f were identified in wastewater, w
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