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Also, the efficiencies various universal high-throughput sequencing 16S rRNA gene primers in anammox bacteria investigations were also assessed to deliver a reference for primer choice. According to our in silico evaluation results, nothing for the 16S rRNA gene primers could recover every one of the known anammox bacteria, but multiple hzo and hzs gene primers could accomplish this task. But, unsure copies (1-3 copies) of hzo genes were identified when you look at the
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