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In this research, we devised an oral HPV detection method making use of mouthwash waste fluids that causes less emotional opposition to browsing outpatient otolaryngology departments. We successfully detected only the certain unique reverse sequencing probe (using pyro-genotyping) and identified the nine genotypes of HPV targeted for vaccination by pyrosequencing the mouthwash waste liquids of non-head and neck cancer client volunteers (n = 52). A relatively high number (11/52) of mouthw
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