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The final engineered strain reached a volumetric titre of 9 mg L-1 culture of bisabolene after growing for 12 days. When the cultures were grown in a high cell density (HCD) system, we observed an increase in the volumetric titres by one order of magnitude for all producing-strains. The strain with improved MEP pathway presented an increase twice as much as the remaining engineered strains, yielding more than 180 mg L-1 culture after 10 days of cultivation. Furthermore, the overexpression of these two MEP enzymes prevented the previousl
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