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The Na+/K+ ratio was not significantly different between the mutant maize plants and WT plants under salt stress conditions, but the Na+ and K+ content was increased in zmcps-1 and zmcps-7 leaves and shoots. Na+ fluorescent dye staining showed that the mutant leaves have significantly higher vacuolar Na+ intensity than the WT maize. The expression level of vacuolar Na+/H+ exchanger gene ZmNHX1 and vacuolar proton pump genes ZmVP1-1 and ZmVP2 were upregulated in the zmcps-1 and zmcps-7 plants under salinity, further proving that in vivo
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