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In this system, AF, serves as an effective energy transfer donor, which can transfer energy to PtNCs and Ru(dcbpy)32+directly. Meanwhile, PtNCs, both as the acceptor and donor, can accept energy from AF and transfer it to Ru(dcbpy)32+. As a result, The biosensor achieves enhanced ECL efficiency, which is 1.78 times that of the classic tris(2,2'-bipyridyl)ruthenium(II) dichloride (Ru(bpy)32+) and exhibits good responses to miRNA-141 in the linear range from 10 aM to 100 nM with a detection limit of 3.3 aM. Also, the obtained biosensor can be employed to dete
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