https://bay2402234inhibitor.co....m/enhancing-the-exac
The molecular mechanisms underlying antianoikis properties in cancer cells are still mainly uncertain. Right here, we explain a protocol for preparation of anoikis-resistant cultured nonmetastatic MCF-7 and metastatic MDA-MB-231 cellular outlines. The anoikis-resistant cultures were served by plating cells when you look at the poly-2-hydroxyethyl methacrylate coated plates and cultured for 24 h. The viability of cells within the countries was determined making use of trypan blue staining and annexin
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