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The emergence of the NDM-1-positive ( ) strains has led to limited therapeutic options for clinical treatment. Understanding the clinical characteristics, antimicrobial resistance, biofilm assay, and the virulence genes of these isolated strains is of great significance. The polymerase chain reaction (PCR) was used to screen isolated NDM-1-positive . The clinical information of the patients was collected from medical records. The NDM-1-positive isolates were subjected to antimicrobial susceptibility testing and multilocus sequence typing
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