https://www.selleckchem.com/pr....oducts/Cyclosporin-A
Overexpression of RFP-tagged proteins in growing tobacco pollen tubes together with the genetically encoded Ca2+ sensor YC3.6 allows to analyze localization and dynamics of the protein of interest, as well as the impact of its overexpression on Ca2+ dynamics and pollen tube growth. Here, we describe a step-by-step instruction for transient transformation of N. tabacum pollen and subsequent in vitro germination and Ca2+ imaging.Live cell imaging at high resolution of pollen tubes growing in vitro requires an experim
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