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In the event of fetal no-cost DNA focus less than 4%, the success rate of getting effective NIPT results by re-sampling and re-detection increases because of the increase of gestational age, but may hesitate the diagnosis for fetal aneuploidies. Therefore, personalized estimation is made based on gestational age and medical indications. It's advocated that expecting mothers should choose unpleasant prenatal analysis once they failed into the retest.With the development of high-throughput
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