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Processing of the simulated data set resulted in a 1.86 Å from 20,214 particles, similar to a 2 Å density map obtained from 29,224 particles selected from real micrographs. Simulated data sets were then generated for a 14 kDa protein, hen egg white lysozyme (HEWL), with and without an ideal phase plate (PP). Whereas we could not obtain a high-resolution 3D reconstruction of HEWL for the data set without PP, the one with PP resulted in a 2.78 Å resolution density map from 225,751 particles. Our simulator and simulations could help in push
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