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INTRODUCTION We investigated the mechanisms underlying immobilization-induced muscle pain in rats. METHODS In rat skeletal muscle, pressure pain threshold (PPT) of the gastrocnemius muscle was measured, and nerve growth factor (NGF) level, peripheral nerve fiber density, macrophage number, and interleukin-1β (IL-1β) mRNA expression were examined. An NGF receptor inhibitor was injected intramuscularly to assess the relationship between PPT and NGF levels. RESULTS Immobilization resulted in a decrease in PPT and increases in NGF level, C-
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