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This work describes the reuteransucrase-catalyzed reaction and structural characterization as well as in vitro fermentation for the acceptor products of gluco-oligomers from sucrose and maltose. At a low concentration of sucrose, the production of gluco-oligomers was favored, resulting in a relatively large number of acceptor products (DP3-5). A mathematical model was also proposed to simulate gluco-oligomer production depending on the reaction conditions. The fine structures of major linear gluco-oligomer fractions for a sucrose malto
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