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In the present research we established a recombinant expression system for barley magnesium chelatase with the lasting goal to acquire architectural information with this enigmatic chemical complex from a higher plant. The genetics Xantha-h, -g and -f were cloned in plasmid pET15b, which permitted manufacturing regarding the three subunits as His-tagged proteins in Escherichia coli BL21(DE3)pLysS. The purified subunits stimulated magnesium chelatase activity of barley plastid extracts a
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