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The obtained estimates are close to the parameter values used in studies of electroporation.We measure the free energy of a model filament, which undergoes deformations and structural transitions, as a function of its extension, in silico. We perform Brownian Dynamics (BD) simulations of pulling experiments at various speeds, following a protocol close to experimental ones. The results from the fluctuation theorems are compared with the estimates from Monte Carlo (MC) simulation, where the rugged free energy landscape is produced by the
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